sybr safe excitation wavelength
<]>> For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Refer to Table 5 ⦠SYBR ® Green displays very low background fluorescence, and its excitation ⦠SYBR Safe stain offers excellent sensitivity in nucleic acid visualization with either UV excitation or blue-light excitation. SYBR ® Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. Urrutia G, Salmonson A, et al. the excitation and emission wavelengths of ethidium bromide (Ex/Em = 302 & 518/605 nm 1,2) and other visible fluorescent nucleic acid stains and provide a sensitive gel documentation option. GelGreen® has green fluorescence, similar to SYBR® Green or SYBR® Safe⦠and SYBR® Safe (Invitrogen Corp.) stains exhibit low mutagenicity and no toxicity as reported by their manufacturers (refer to safety information on manufacturerâs Web sites). 4. x�b```f``Z������ Ā B�@Q��, ,, The SmartBlue can also works for excitation ⦠N�,�"֩ �Gw2D�b�v``�\�����%Hnz �nU�� �S�k�$'��bTbf��f�iI�J?���ǸbX���p\�d��s�)�U���B�$�a���m. Filter recommendations for use With SYBR Safe ⦠Thus, 254 nm or 300 nm UV-excitation will work, as will 488 nm lasers, 470 nm ⦠0000002880 00000 n SYBR Safe stain can be added into an agarose gel for staining during electrophoresis or the gel can be incubated in SYBR Safe stain solution following electrophoresis. 0000007514 00000 n 2. SYBR Nucleic Acid Gel Stains Introduction Since their introduction in late 1993, our SYBR ® nucleic acid gel stains have rapidly become very popular due to their high sensitivity and ease of use. GelRed® has red fluorescence, similar to ethidium bromide. When bound to nucleic acids, the green-fluorescent SYBR Safe stain has fluorescence excitation max at ~280 and ~502 nm, and an emission max at ~530 nm. When used on the E-Gel Power Snap Electrophoresis Device with integrated blue-light trans-illuminator, this enables safe ⦠0000002201 00000 n The basic information on the use of each SYBR ⦠You can further decrease your exposure risk by using visible blue-light illumination with SYBR Safe stain rather than UV illumination. The arrangement of SYBR green stained nuclei in the center of PNCs along with auto-fluorescence of the chloroplasts. Get updates, discounts and special offers. Samples stained with SYBR Safe® (during gel electrophoresis or as a post run stain) can be easily detected by the MicroBIS imaging system. Example compatible stains include: SYBR Green, GelGreen, SYBR Safe, SYBR ⦠E-Gel agarose gels are pre-stained with SYBR Safe DNA gel stain which has an excitation wavelength in the blue-light spectrum. SYBR Green I is the most frequently used dsDNA-specific dye in qPCR. Volkman HE, Cambier S, et al. Tel: +1-832-696-8203Fax: +1-832-641-3177 Email: [email protected]. SYBR Safe DNA Gel Stain has two main excitation peaks: in the UV region at 280 nm, and in the visible region at 502 nm. Bound to nucleic acids, SYBR Safe stain has fluorescence excitation maxima at 280 and 502 nm, and an emission maximum at 530 nm. A - SYBR Safe stain has two main excitation peaks: in the UV at 280 nm, and in the visible region at 502 nm. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). startxref 0000001502 00000 n Example compatible stains include: SYBR Green, GelGreen, SYBR Safe, SYBR ⦠The fluorescence of both Ethidium bromide and SYBR is well visible with 365 nm transilluminator. SYBR Safe stain is supplied as a concentrate solution that can be used like an ethidium bromide solution. SYBR Safe ⦠Safer for samples, safer for you: The SmartBlue Transilluminator is the perfect partner for the most common safe DNA stains. Donât worry about filter, since the excitation wavelengths for both dyes far exceed even 365 â¦
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